Abstract
In order to treat the growing number of antibiotic resistant pathogens, the isolation and production of new antibiotics is necessary. The need to discover novel antibiotics is increased by the lack of interest of large pharmaceutical companies. The three bacterial strains being studied herein were isolated from the phytotelma of Serracenia pitcher plants found in Western North Carolina, or from soils collected on public lands in Utah, U.S. Pseudomonas CM/CP G1 was grown in a 25 mM sodium succinate minimal media, Streptomyces SS568 was grown in both glucose and acetate minimal medias (12.5mM), and SS827 was grown in 12.5 mM citrate minimal media. Antibiotic activity of crude extracts were tested against Gram-positive Staphylococcus aureus (grown on 10% Triptic Soy Agarose plates). The presence of antibiotic compounds has been confirmed for all strains. Compounds isolated via column chromatography from SS568 and SS827 were probed for antibiotic activity against S. aureus. Structural characterization of active compounds using 1D and 2D NMR, IR, and LC-MSMS are ongoing.
How to Cite
Thurman, A. M., (2017) “Antibiotic Activity of Natural Products Produced by Bacteria Isolated in North Carolina and Utah”, Capstone, The UNC Asheville Journal of Undergraduate Scholarship 30(1).
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