Abstract
There are large bodies of evidence linking the G?12/13 family of G proteins to oncogenic signaling and tumor development. G?12 and G?13 contribute to the development of cancer through several avenues including regulating the expression of several proteins through a series of transcription factors. One gene previously unknown to be affected by G?12/13 is calr. The protein product of this gene, calreticulin, is linked to cancer development in part because of its demonstrated ability to negatively regulate cellular adhesion through transcriptional regulation of e-cadherin. Data from protein separation and immunoblotting experiments demonstrate increased calreticulin expression predominantly in the nuclear and mitochondrial fractions of human embryonic kidney cells made to express wild type and constitutively activated forms of G?12 and G?13. This suggests that G?12 and G?13 may regulate the expression of calreticulin, a protein previously unknown to be affected by G?12 or G?13. Future work will examine the mechanism behind this newly discovered relationship and its effects on the expression of epithelial-cadherin and loss of cellular adhesion.
How to Cite
Durall, R. T., IV, (2021) “G?12/13 Regulate the Expression of Calreticulin”, Capstone, The UNC Asheville Journal of Undergraduate Scholarship 34(2).
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